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            血液細胞重編程iPS細胞建系

             人多能干細胞研究  目錄

            hiPS細胞建系

            專用培養基  個性化服務  7×24h技術支持

            為您實現將疾病模型培養在培養皿中的理想

            Generation of Integration-Free iPS Cells From 5ml Human Blood


            如何取的合適的樣本?

            如何防止樣本被污染?

            實驗室實驗條件是否能夠滿足?

            如何高效率重編程生成hiPS細胞?

                      。。。。。。。。。。???

            還在為取材困難而無法建立疾病模型煩惱嗎?我們可利用Episomal  iPSC Reprogramming高效率重編程系統將5-10 mL外周血經轉染后即可誘導生成無外源基因插入整合的hiPSCs,為您輕松建立理想的疾病模型,用于iPSCs疾病模型的建立、藥物開發、細胞治療以及其它再生醫學研究領域。


            適合應用領域:

            1. iPS疾病模型的建立

            2. 創傷樣本無法取材

            3. 疾病模型用于藥物開發

            4. 細胞治療以及再生醫學研究

            5. 尿液樣本量少,無法滿足實驗需求

            from blood to iPS.png


            取材簡便:5-15 mL血即可完成重編程建系。

            實驗周期短:從人外周血到iPS細胞系建成只需3-4周。

            無基因整合:采用Episomal系統,無外源基因插入整合。

            重編程效率高:重編程效率高,提供完整的iPS檢測報告。

            hiPSCs細胞鑒定:

            iPS.png


            載體信息:

            pEV-SFFV-OCT4-Wpre; 

            pEV-SFFV-SOX2-Wpre; 

            pEV-SFFV-Myc-Wpre; 

            pEV-SFFV-KLF4-Wpre; 

            pEV-SFFV-OCT4-E2A-SOX2-Wpre;

            pEV-SFFV-MYC-E2A-KLF4-Wpre; 

            pEV-SFFV-BCL-XL-Wpre


            注:

            SFFV: spleen focus-forming virus U3 promoter; 

             E2A: a self-cleavage site derived from equine rhinitis A virus,(CAG TGT ACT AAT TAT GCT CTC TTG AAA TTG GCT GGA GAT GTT GAG AGC AAC CCA GGT CCC); 

            WPRE: posttranscriptional regulatory element; Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed, creates a tertiary structure enhancing expression. The sequence is commonly used in molecular biology to increase expression of genes delivered by viral vectors.

            SV40PolyA: polyadenylation signal from SV40 virus; 

            OriP: EBV origin of replication; 

            EBNA1: Epstein–Barr nuclear antigen 1, which plays essential roles in replication and persistence of episomal plasmid in infected cells.



            請聯系諾為生物,www.novobiotec.com    support@nwbiotec.com  電話:010-57438396

             

            參考文獻:

            1. Meng X, Neises A, Su RJ, Payne KJ, Ritter L, Gridley DS, Wang J, Sheng M, Lau KH, Baylink DJ, Zhang XB: Efficient reprogramming of human cord blood CD34+ cells into induced pluripotent stem cells with OCT4 and SOX2 alone. Molecular Therapy, 2012;20(2):408-16.


            2. Su RJ, Baylink DJ, Neises A, Kiroyan JB, Meng X, Payne KJ, Tschudy-Seney B, Duan Y, Appleby N, Kearns-Jonker M, Gridley DS, Wang J, Lau KHW, Zhang XB: Efficient generation of integration-free iPS cells from human adult peripheral blood using BCL-XL together with Yamanaka factors. PLoS One. 2013, 8(5): e64496.


            3. Zhang XB: Cellular reprogramming of human peripheral blood cells. Genomics Proteomics Bioinformatics. 2013 Oct;11(5):264-74.


            4. Su RJ, Neises A, Zhang XB: Generation of iPS cells from human peripheral blood mononuclear cells using episomal vectors. Methods Mol Biol. 2016;1357:57-69.


            5. Wen W#, Zhang JP#, Xu J#, Su RJ, Neises A, Ji GZ, Yuan W, Cheng T*, Zhang XB*. Enhanced generation of integration-free iPSCs from human adult peripheral blood mononuclear cells with an optimal combination of episomal vectors. Stem Cell Reports. 2016 Jun 14;6(6):873-84.


            6. Wen W, Zhang JP, Chen W, Arakaki C, Li XL, Baylink D, Botimer GD, Xu J, Yuan W, Cheng T*, Zhang XB*. Generation of integration-free induced pluripotent stem cells from human peripheral blood mononuclear cells using episomal vectors. J Vis Exp. 2017 Jan 1;(119). doi: 10.3791/55091.


             人多能干細胞研究  目錄

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